Fig. 3

Adhesion measurements in CDX2 inducible LS174T cells a. CDX2 protein expression was compared using western blotting. Cell lysate from LS174T wild type cells and LS174T cells with inducible CDX2 with or without doxycycline treatment was used in the analysis. Vinculin was used as a control. Bands are from the same gel b. The Cell Index for CDX2 negative LS174T cells seeded in pre- and postoperative serum was measured. Mean results at 60 min for pre- and postoperative cell adhesion for each patient is shown. n.s. = not significant. c. The difference in percentage in adhesion at 60 min was calculated for each patient. The positive bars (black) indicate patients with higher adhesion in postoperative compared to preoperative serum, while the negative bars (grey) indicate patients with higher adhesion in preoperative compared to postoperative serum. d. The Cell Index for CDX2 positive LS174T cells seeded in pre- and postoperative serum was measured. Mean results at 60 min for pre- and postoperative cell adhesion for each patient is shown. ****p < 0.0001. e. The difference in percentage in adhesion at 60 min was calculated for each patient. The positive bars (black) indicate patients with higher adhesion in postoperative compared to preoperative serum, while the negative bars (grey) indicate patients with higher adhesion in preoperative compared to postoperative serum