Fig. 1
Generation and characterization of a DZNep-resistant clone. a Scheme of the generation of the DZNep-resistant clone and its control. b Comparison of the apoptotic response of BLUE-1 K10 (control) and BLUE-1R10 to DZNep. Above: The cell lines BLUE-1R10 and BLUE-1 K10 were either treated with 5 μM DZNep or untreated for 72 h. Cells were harvested and the percentage of apoptotic cells was determined by flow cytometry. Data is shown as mean plus standard deviation (SD) of three biological replicates. Below: Western blot analysis was performed using total protein lysates from both cell lines either untreated or treated with 2 μM and 5 μM DZNep, respectively. GAPDH was used as a loading control for the Western blot. The full-length blot is presented in Additional file 6: Fig. S5. The FUSION-CAP Software was used for Western blot image analysis. c Comparison of the doubling time of BLUE-1, BLUE-1 K10 and BLUE-1R10. The three cell lines were cultivated at a seeding density of 2 × 105 cells in 6-well culture plates. The number of vital cells were measured at 24 h, 48 h and 72 h by flow cytometry. Doubling time is shown as mean plus SD of triplicate measurements. ns: not significant