Fig. 3

ALDH1A3 knockout facilitated luminal cells resistance to ADT therapy. a: Western blot validation for two candidate guides RNA of Cripsr-cas9, full-length blots are presented in Supplementary Figure 1–2. b: The cell numbers reflecting cell growth curve for different groups, sgGFP (control cells in normal medium), sgGFP+DHT(−) (control cells in charcoal stripped medium), sgALDH1A3 (Crispr-cas9 knockout ALDH1A3 cells in normal medium), sgALDH1A3 + DHT(−) (Crispr-cas9 knockout ALDH1A3 cells in charcoal stripped medium). Both in LnCaP and VCaP cells, there is significant difference between sgALDH1A3 and sgGFP in charcoal stripped medium at day 14 (p < 0.01). c: Morphologic observation for different groups