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Fig. 2 | BMC Cancer

Fig. 2

From: Bis-benzylidine Piperidone RA190 treatment of hepatocellular carcinoma via binding RPN13 and inhibiting NF-κB signaling

Fig. 2

RA190B binds to RPN13 in HepG2 cells. a HepG2 cell lysates were incubated with 0, 5, 10 or 25 μM RA190B, separated by SDA-PAGE, transferred to a membrane and probed with HRP-streptavidin. After development using chemiluminescence, a prominent band at 42 KDa was evident, a molecular weight consistent with RPN13 (full-length blots/gels are presented in Fig. S6). b RPN13 (ADRM1) mRNA expression as determined by quantitative RT-PCR was significantly increased after 4 h RA190 treatment

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