Fig. 5
From: A novel pancreatic tumour and stellate cell 3D co-culture spheroid model
Spheroid cultures used for virtual sorting. Bright field (a) and combined bright field fluorescence photographs (b) of mono- and heterospheroids from day 5 of the indicated cell lines are shown. Panc1 and HPAFII are human tumour cell lines, mPSC is an immortalized mouse pancreatic stellate cell line. KPCT is a pancreatic cancer cell line established from a KrasLSL-G12D/+;Trp53LSL-R172H/+;Pdx-Cre (KPC) mouse mated to a mouse with the tdTomato allele (B6.Cg-Gt (ROSA)26Sortm9(CAG-tdTomato)Hze/J) and hPSC is a human pancreatic stellate cell line. Virtual sorting real time PCR shows cell type- and species-specific mRNA expression in Panc1 and mPSC mono- and heterospheroids. The mRNA expression of Asma (Acta2), Col1a1, Fn and TGFβ1 (c) and MKI67 and CDH1 (d) are depicted over a time period of 7 days. The values were normalized to day 3 mPSC- (c) and Panc1 mono-spheroids/−cultures (MC) (d), respectively. p-values were as follows: *, p < 0.05; **, p < 0.01. Black scale bars in a) and b) correspond to 200 μm