Fig. 3
From: A novel pancreatic tumour and stellate cell 3D co-culture spheroid model
Immunohistochemical characterisation and expression analyses of Panc1/hPSC mono- and heterospheroids. Immunohistochemical staining for WT1 (Wilms tumour 1), VIM (vimentin) and CD10 (MME, membrane metalloendopeptidase) of Panc1 and hPSC mono- and heterospheroids cultured for 5 days (a). Normalized mRNA expressions of ASMA (ACTA2, actin alpha 2, smooth muscle), COL1A1 (collagen 1 type A1), FN (fibronectin) and TGFβ1 (transforming growth factor beta 1) are shown from spheroid cultures at the indicated time points. All values have been normalized to the expression of the individual genes in Panc1 mono-cultures at day 3 (b). Representative example of an E-cadherin (CDH1) protein staining (brown colour) from day 5 spheroids and the detailed quantification of CDH1-positive cells over a time period of 7 days (c). Black scale bars in a) and c) correspond to 100 μm