Fig. 2

Silencing of DANCR reduces the malignant behaviors of CC cells. si-DANCR plasmids were transfected into HT29 and SW116 cells with scramble siRNA as NC. a, DANCR expression following si-DANCR-1 and si-DANCR-2 plasmid transfection detected using RT-qPCR; b, proliferation of HT29 and SW116 cells measured via EdU assay; c, viabilities of HT29 and SW116 cells detected using MTT assay; d-e, apoptosis of HT29 and SW116 cells evaluated using Hoechst 33258 staining (D) and flow cytometry (E); f, protein levels of EMT markers Snail, Vimentin and E-cadherin in cells determined by western blot analysis (See original images in Supplementary Figure S1); g, invasion and migration abilities of HT29 and SW116 cells assessed via Transwell assays. Data are expressed as mean ± SD; in panels A, B, D, E and F, data were analyzed using one-way ANOVA, while data in panels C and F were analyzed via two-way ANOVA, and Tukey’s multiple comparison test was applied for the post hoc test; *, p < 0.05