Table 2 Primer sequences
Gene | Forward primer 5′-3′ | Reverse primer 5′-3′ | Amplicon size | GenBank Accession no. |
|---|---|---|---|---|
OCT4 (POU5F1) | TACTCCTCGGTCCCTTTCC | CAAAAACCCTGGCACAAACT | 166 bp | NM_002701 |
NANOG | TGATTTGTGGGCCTGAAGAAAA | GAGGCATCTCAGCAGAAGACA | 60 bp | NM_024865.4 |
NODAL | AGCATGGTTTTGGAGGTGAC | CCTGCGAGAGGTTGGAGTAG | 160 bp | NM_001329906.1 |
CRIPTO | TCCTTCTACGGACGGAACTG | ATCACAGCCGGGTAGAAATG | 153 bp | NM_001174136.1 |
LEFTY1 | GCCTCGACAGTGCATCGCCTC | CAAGTAAACAATGACACATTGGGC | 477 bp | NM_020997.4 |
RPS20 | AGACTTTGAGAATCACTACAAGA | ATCTGCAATGGTGACTTCCAC | 179 bp | NM_001023 |
RPS29 | CGCTCTTGTCGTGTCTGTTCA | CCTTCGCGTACTGACGGAAA | 91 bp | NM_001032 |