Table 1 Antibody dilutions, retrieval buffer and details. Antigen-retrieval buffers: citrate buffer, 10 mM, pH 6.0; TEG buffer, 10 mM Tris, 0.5 mM EGTA, pH 9.0
Antibody | Dilution | Retrieval buffer | Species | Company | Cat. Number |
|---|---|---|---|---|---|
OCT4 | 1:50 | TEG | Mouse | Santa Cruz | Sc-5279 |
NODAL | 1:800 | Citrate | Mouse | Abcam | Ab55676 |
CRIPTO | 1:200 | Citrate | Rabbit | Abcam | Ab19917 |
LEFTY | 1:4000 | Citrate | Rabbit | Abcam | Ab22569 |
cPARP | 1:100 | Citrate | Rabbit | Cell Signaling | 5625 |
BrdU | 1:100 | Citrate | Mouse | Dako | M0744 |