Fig. 6

Passive permeability changes of PC-9-Br treated with cisplatin+pemetrexed. (a) A representative cresyl violet brain slice (approximately corresponding to Fig. 2 C3) of cisplatin+pemetrexed-treated PC-9-Br tumors, with (b) corresponding Tomato Red tumor fluorescence. c The same slice with Oregon Green, d ICG, and e¹⁴C-AIB autoradiographic data to quantify P-gp, vascularity, and permeability increases, respectively. f The median and interquartile ranges for fold-increases of passive permeability markers in 96 tumors over control regions. For cisplatin-pemetrexed-treated brains, tumors were significantly more permeable to ¹⁴C-AIB (red) and OG (green) (p < 0.05), but not ICG (blue) (p > 0.05). (G) While the OG (green) intensity had a modest correlation with mm² (r² = 0.42), ICG (blue) and ¹⁴C-AIB (red) were not correlated with metastases size (r² < 0.15). For all depicted brain slices, tumor regions are outlined while control areas are squares. Scale bar = 1 mm