Fig. 4

Permeability changes of PC-9-Br treated with vehicle. a A representative cresyl violet brain slice of vehicle-treated PC-9-Br tumors, with b corresponding Tomato Red tumor fluorescence. c The same slice with Oregon Green, d ICG, and e¹⁴C-AIB auto-radiographic data to quantify permeability increases. f The median and interquartile ranges for fold-increases of passive permeability markers in 114 tumors over control regions. For vehicle brains, tumors were significantly more permeable to OG (green) and ¹⁴C-AIB (red) (p < 0.05), but not ICG (blue) (p > 0.05). g The fold increases of OG (green), ICG (blue), or ¹⁴C-AIB (red) were not correlated with metastases size (r² < 0.02). For all depicted brain slices, tumor regions are outlined while control areas are squares. Scale bar = 1 mm