Table 2 Comparisons of IDH1/2 mutations in ICCs between DNA sequencing and IHC methods
DNA sequencing | MsMab-1 staining | |||||
|---|---|---|---|---|---|---|
Mutant gene | Nucleotide change | Amino acid change | Number | Positve | Negative | Reactivity by manual |
IDH1 | CGT > TGT | R132C | 6 | 1 | 5 | No |
IDH1 | CGT > GGT | R132G | 5 | 4 | 1 | Yes |
IDH1 | CGT > CAT | R132H | 4 | 4 | 0 | Yes |
IDH1 | CGT > CTT | R132L | 2 | 1 | 1 | No |
IDH2 | AGG > TGG | R172W | 2 | 1 | 1 | Yes |
IDH2 | AGG > AAG | R172K | 2 | 0 | 2 | No |
Wild type | Â | Â | 109 | 3 | 106 | No |