Fig. 3

ELAVL1 is a downstream target gene of miR-514a-5p in NPC. a The downstream mRNAs of miR-514a-5p were obtained from starBase website. b RNA pull down assay explored the interaction between miR-514a-5p and its downstream mRNAs. c The expression of ELAVL1 in NPC cells and nasopharyngeal epithelial cell line was investigated by RT-qPCR. d It was manifested by RIP assay that ELAVL1 and miR-514a-5p coexisted in RISC. e The influence of miR-514a-5p overexpression on ELAVL1 mRNA and protein level was revealed by RT-qPCR and western blot assay respectively. These blots were cropped. The uncropped blots were shown in Supplementary figure 2. f The putative binding sites of ELAVL1 and miR-514a-5p, as well as the mutant sites in mutant-type ELAVL1 reporter were illustrated. g The binding ability between ELAVL1 and miR-514a-5p was proofed by luciferase reporter assay. GAPDH was used as an internal control. Error bars represent the mean ± SD of at least three independent experiments. ^**P < 0.01, ^***P < 0.001