Fig. 5

lncRNA CRNDE/miR-539-5p/POU2F1 involved in NF-κB and AKT pathway activity. We divided the cells into groups: pcDNA3 + siPOU2F1, pcDNA3 + siNC, pcDNA3/ lncRNA CRNDE+siPOU2F1, lncRNA CRNDE+siNC, transfected for 48 h. Western blot was performed to detect the effects of lncRNA CRNDE/miR-539-5p/POU2F1 on IKBa(a), AKT(b), ERK(c) phosphorylation level. d The NF-κB(p65) level in nuclear was detected when cells were treated with lncRNA CRNDE/miR-539-5p/POU2F1. *p < 0.05, **p < 0.01. All bars indicate the means±SD. All experiments were performed independently at least three times