Fig. 5
From: HOXB4 promotes the malignant progression of ovarian cancer via DHDDS
Cell function analysis of the effect of HOXB4/DHDDS in OV. a Western blot analysis was performed to detect the protein levels of DHDDS after treatment. b Colony formation assays detected that overexpression of HOXB4 and DHDDS promoted the proliferation of OVCAR3 cells, and knockdown by shRNA suppressed the proliferation of SKOV3 cells. HOXB4 or DHDDS knockdown by shRNA suppressed cell proliferation, while DHDDS overexpression in SKOV3 cells elicited neutralizing effects. c Wound-healing assay. d Invasion assay revealed results similar to those in A. HOXB4 transcriptional activation of DHDDS to promote cell progression. Statistically significant differences were analyzed using one-way ANOVA. Statistically significant differences of luciferase activity assay between two groups were analyzed using Student’s t-test, and Pearson correlation analysis was analyzed Pearson Chi square test