Fig. 4

Motifs recognized by HOXB4 in its target DHDDS. a Motif analysis of the promoter region of the target gene DHDDS by using ChIPseeker. Two individual motifs and their locations within the promoter of DHDDS, Chr1 629,791–630,040 and Chr1 633,904–634,132, respectively. b Western blot was employed to detect the level of DHDDS after HOXB4 knockdown and overexpression; HOXB4 promoted DHDDS expression (P < 0.05). c Luciferase reporter assay showed that HOXB4 transcriptionally activated motifs 1 and 2, and the effect was enhanced by the 1 + 2 sequences together (P < 0.05). d TCGA analysis showed a correlation between HOXB4 and DHDDS expression in the OV samples. e The correlation between HOXB4 and OV patient survival time. Statistically significant differences were analyzed using Student’s t-test