Fig. 3

HOXB4 facilitated OV cell proliferation by promoting progression. a Protein level of HOXB4 in different OV lines. b Efficiency of HOXB4 knockdown and overexpression in SKOV3 and OVCAR3 cells, respectively. c & d Wound healing assay to detect the effect of HOXB4 in OVCAR3 and SKOV3 cells (P < 0.05, respectively). e & f A cell colony formation assay was used to examine the effect of HOXB4 on the proliferation of OV cells. HOXB4 promoted the proliferation of OV cells (P < 0.05). g & h Analysis of invasion by Transwell assay in OVCAR3 and SKOV3 cells. HOXB4 accelerated the invasion of OV cells (P < 0.05). i & j Zymography assays (i) and Western blot (j) to detect invasion and EMT marker expression levels after HOXB4 knockdown or overexpression. Statistically significant differences were analyzed using Student’s t-test