Fig. 3
The NLRP3 inflammasome and GSDMD are increased in endometrial cancer cells. a Western blotting analysis revealed that levels of NLRP3, ASC, pro-caspase-1, caspase-1, and GSDMDNterm protein in Ishikawa and HEC1A cells were over-expressed after treatment with hydrogen-supplemented culture medium (H-CM) after 24 h compared with the levels in normal culture medium (CM) or in endometrial epithelial cells. b Expression of caspase-1 in Ishikawa, HEC1A, and AN3CA cells was significantly increased in the H-CM group compared with the level in the CM group. c Expression of NLRP3, pro-caspase-1, caspase-1, and GSDMDNterm was increased in hydrogen-treated AN3CA and HEC1A cells and decreased in the NAC treated H-CM group. Relative optical densities are shown beside each group. Data are expressed as mean ± S. d * P < 0.05, # P > 0.05 compared with the control group. Each experiment was performed in triplicate (See Additional file 5)