Table 1 Immunohistochemical protocols
Antigen | Clone and origin | Dilution, incubation time | Source, reference | Antigen retrieval | Detection system |
|---|---|---|---|---|---|
ERα | C311 mouse mAb | 1:50 44 min | Santa Cruz Biotechnology, sc-787 | None | iView DAB detection kit (Ventana Medical Systems, Roche Diagnostics, 760–091) |
PR | 10A9 mouse mAb | 1:50 1 h 40 min. | Meridian Life Science, K42546 M | HIER, CC1, 1 h | iView DAB detection kit |
HER2 | 4B5 rabbit mAb | Prediluted 8 min | Roche Diagnostics, 790–2991 | HIER, CC1, 30 min | UltraView Universal DAB detection kit (Ventana Medical Systems, Roche Diagnostics, 760–500) |
Ki-67 | MIB1 mouse mAb | 1:50 32 min | Dako, M7240 | HIER, CC1, 1 h | iView DAB detection kit |
Cytokeratin 14 | LL002 mouse mAb | 1:150 44 min | Santa Cruz Biotechnology, sc-58,724 | HIER, CC1, 56 min | iView DAB detection kit |
AR | SP107 rabbit mAb | 1:400 1 h 20 min. | Spring, M4070 | HIER, CC1, 30 min | OptiView DAB IHC Detection Kit (Ventana Medical Systems, Roche Diagnostics, 760–700) |
FOXA1 | SP88 rabbit mAb | 1:50 1 h 20 min. | Spring, M3884 | HIER, CC1, 56 min | OptiView DAB IHC Detection Kit |