Fig. 1

miRNAs differentially expressed in CRC modulate proliferation of HCT116 cells. HCT116 cells were transfected with 31 miRNA mimics and a control miRNA, cultured for 4 days in 96-well plates, stained with propidium iodide (PI) and Hoechst 33342, and submitted to quantitative automated fluorescence microscopy. Graph shows total cell numbers (Hoechst segmented nuclei) as blue circles and the percentage of dead cells (PI+) as red triangles. Data is expressed as mean ± SD and symbols in gray indicate no statistically significant differences (P < 0.05, unpaired one-tailed non-parametric Mann-Whitney test; n = 4 replica wells, 9 sites/well). The dotted line indicates the mean value obtained for the controls (black symbols). Differential expression in CRC, as found in the literature (Table 1), was used to group miRNAs and is indicated at the bottom of the figure