Fig. 4
From: Loss of Stag2 cooperates with EWS-FLI1 to transform murine Mesenchymal stem cells
Anchorage-independent growth in soft agar after Stag2 knockdown. Representative plates are shown for a Ctrl shRNA+10Gy cells and b Stag2 shRNA+10Gy cells. c The mean number of colonies per plate was 908 (95% CI 744–1072) for Stag2 shRNA+10Gy cells and 520 (95% CI 422–618) for Ctrl shRNA+10Gy cells (p < .001). d Digital image analysis to determine colony size by pixels showed a mean size of 4.6 pixels/colony (95% CI 3.9–5.4) for Ctrl shRNA+10Gy cells compared to 8.8 pixels/colony (95% CI 7.4–10.3) for shRNA+10Gy cells (p < .001). Assays were done in triplicate