Fig. 2

a Differential interference contrast from multispectral microscope of PANC1 stained cells at 13 h, b. Example multispectral channel 25 showing autofluorescence at excitation 432 nm emission 593 nm, c. Same field of view after staining with Dapi, taken using the confocal microscope, d. Same field of view after staining for PCNA, taken using confocal microscope. Regions of interest (cell area) were defined using (a), cell cycle phase was determined through measurement of fluorescence intensity in (c) and PCNA pattern in (d), then matched to data from multispectral channels including (b)