Fig. 5

Numbl increases Integrin β1 expression and promotes myeloma cell adhesion. a RPMI 8226 and H929 cells were transfected with a Numbl-expressing plasmid or a control plasmid, or Numbl-specific or control siRNA plasmids for 48 h. The change in the expression of Integrin β1 was detected by Western Blot analysis (up), Western Blot results in gray value indicate quantification (down). * compared with control group (HA-Numbl compared with HA group and RNAi compared with Ctrl group, respectively), P < 0.05. b-c RPMI 8226 and H929 cells were transfected with Numbl-expressing plasmid or control plasmid, or Numbl-specific or control siRNA plasmids for 48 h. Cell adhesion to FN (b) or HS-5 (c) cell-coated plates was then analyzed by Calcein-AM cell adhesion assay. *, # compared with the control group (HA-Numbl compared with HA group and RNAi compared with Ctrl group, respectively), P < 0.05