Fig. 2

Invadopodia formation can be suppressed by co-culturing PaTu8902 and CFPAC-1 cells with monocyte-like cells. a Representative images from a PaTu8902 invadopodia assay where the cells were either incubated with control medium (control), incubated with THP1 conditioned medium (THP1-CM), or were cultured with THP1 cells during the invadopodia assay (THP1-DC), or cultured with THP1 cells prior to the invadopodia assay (THP1-PC). For the THP1-PC condition, growth medium (GM) containing the THP1 cells was evacuated and washed with PBS. b Quantification of degradation per field of view for experimental conditions described above (a). c Representative images from a CFPAC-1 invadopodia assay where the cells were either incubated with control medium (control), incubated with THP1 conditioned medium (THP1-CM), or were cultured with THP1 cells during the invadopodia assay (THP1-DC), or cultured with THP1 cells prior to the invadopodia assay (THP1-PC). d Quantification of degradation per field of view for experimental conditions described above (c). e Representative images from a PaTu8902 spheroid assay where the cells were either incubated with control medium (control) or incubated with THP1 conditioned medium (THP1-CM) at 0 h and 48 h. f Quantification of spheroid invasion for experimental conditions described above (e). g Quantification of relative spheroid growth after 48 h for experimental conditions described above (e). h Representative images from a Panc1 spheroid assay where the cells were either incubated with control medium (control) or incubated with THP1 conditioned medium (THP1-CM) at 0 h and 48 h. i Quantification of spheroid invasion for experimental conditions described above (h). j Quantification of relative spheroid growth after 48 h for experimental conditions described above (i). In all cases **** = p < 0.0001, *** = p < 0.001, ** = p < 0.01, * = p < 0.05. Experiments were repeated three times