Fig. 7

In vitro functional characterization of DHT and palmitate (PA) stimulation on AR positive and negative PCa cells. a Proliferation assessed in C4–2B by MTS assay upon PA (range 50–400 μM) or vehicle (control) stimulation for 48 h. b-d Representative bright field images of cultured cells under indicated experimental conditions. e Proliferation assessed in PC-3 M-Pro4 by MTS assay upon PA (range 50–400 μM) or vehicle (control) stimulation for 48 h. f-h Representative bright field images of cultured cells upon experimental conditions. i-l Relative expression or AR responsive genes upon dihydrotestosterone (DHT) or control (EtOH) stimulation, and palmitate (PA) or vehicle (BSA fatty acid free-FFA) or combination (PA + DHT) in AR positive C4–2 and (M-P) LNCaP cells. P-value indicated in the plots is relative to ANOVA. Multiple comparison significance between experimental condition is indicated by * (** p < 0.01, *** p < 0.001). Details related to quantification and normalization are included in the Methods section