Fig. 7

Real-time analysis of MeV-GFP-mediated oncolysis of HT1080 sarcoma cells after co-treatment with PBMC/NKAES isolated from a healthy donor. At 24 h after seeding, HT1080 cells were infected with MeV (MOI 5) (A, lower panel) or mock-infected (a, upper panel; base line controls). At 23 hpi, NK cells from a healthy donor were added at E:T ratios of 1:1, 2.5:1 and 5:1. Triton X-100 was added as a negative control inducing maximum lysis of tumor cells. Real-time cell proliferation was monitored using the xCELLigence RTCA SP system until 72 hpi. Measured electrode impedance is expressed as Cell Index. One representative of two independent experiments performed in quadruplicates using different donors is shown. (b) Statistical analysis of the same experiment. * p < 0.05; ** p < 0.01, *** p < 0.001, n.s. not significant