Fig. 5

Real-time analysis of MeV-GFP-mediated oncolysis of A673 sarcoma cells after co-treatment with PBMC/NKAES isolated from a healthy donor. At 21 h after seeding, A673 cells were infected with MeV (MOI 0.5) (right panels) or mock-infected (left panels; base line controls). At 51 hpi, (a) PBMC, (b) PBMC stimulated with IL-2, or (c) NKAES from a healthy donor were added at an E:T ratio of 2.5:1. Triton X-100 was added as a negative control inducing maximum lysis of tumor cells. Real-time cell proliferation was monitored using the xCELLigence RTCA SP system. Measured electrode impedance is expressed as Cell Index. One representative of three independent experiments performed in triplicates using different donors is shown