Fig. 3

SDC-1 suppressed cell migration and promoted cell adhesion of human colorectal carcinoma cells. pcDNA3.1 or pc-SDC-1 was transfected into SW620 and SW480 cell lines. a Cells were seeded in transwell chamber. Migrating cells were stained and were counted under a microscope. b Substrate fibronectin proteins were coated, and cell adhesion was measured. c-d Western blot analysis was utilized to evaluate the expression levels of MMP-9 and ICAM-1. β-actin was used as an internal reference for normalizing the protein expression. ***p < 0.001