Gankyrin knock-down effect on TP53 and apoptosis. a TP53 pathway showing genes of interest in this study. Relative (b) TP53 (n = 4), (c) P21 (n = 4), (d) BAX (n = 4), (e) FAS (n = 4), (f) BAI-1 (n = 6), (g) PAI-1 (n = 7) mRNA expression in NTera2 cells after Gankyrin knock-down. Data (b-g) analysed by paired t-test, means ±SEM, *p < 0.05, **; p < 0.001. Each data point represents the mean of an individual experiment, each with 3 replicates. Paired samples from an individual experiment are represented by the same colour. H) Effects of Gankyrin knock-down on apoptosis in NT2 cells using Nucview apoptosis (CC3) probe. Representative images of four separate experiments, each with 3 replicates.