FIG. 4
From: EZH2 upregulation by ERα induces proliferation and migration of papillary thyroid carcinoma
ERα contributes to the increase of EZH2 in PTC cells. a ERα and ERβ expression levels were examined by Realtime-PCR in PTC and paired normal thyroid tissues. b ERα and ERβ expression in K1, W3 and Nthy-ori 3–1 cells were analyzed by Realtime PCR. c ERα expression was examined by IHC. Original magnification: × 100 (upper) and × 400 (lower). d Western blot analysis of EZH2 expression in K1 and W3 cells infected with shERα in the presence or absence of 10 nM E2. e K1 and W3 cell lines infected with shERα in the presence or absence of 10 nM E2 and 2 μM GSK126, and then were analyzed by CCK8. f Relationship of relative EZH2 and ERα mRNA expression. Data represent the mean ± S.E.M. *P < 0.05