Fig. 2
From: Role of protein arginine methyltransferase 5 in group 3 (MYC-driven) Medulloblastoma
Expression and correlation of PRMT5 with MYC protein in medulloblastoma cell lines and primary tumors. a Western blotting of PRMT5 in medulloblastoma cell lines with and without MYC amplification compared to normal cerebellum. Actin was used as a loading control. b Comparison of PRMT5 expression levels normalized to Actin in non-MYC vs MYC amplified medulloblastoma cell lines. Significance, p < 0.01. c Representative immunohistochemical images showing PRMT5 and MYC expression and their localization pattern in normal pediatric cerebellum and Group 3 medulloblastoma tumor tissues at 20x magnification. Scale bar, 200 μm. The box plots on right side showing quantification of composite score-based intensity of MYC and PRMT5 staining in Group 3 medulloblastoma tumor specimen (n = 6) compared to normal pediatric cerebellum (n = 4)