Fig. 3

Protein and mRNA expression of SMAD4 after the transfection of cells with plasmids. a, b Representative western blot images of SW1990 and PANC-1 cells. GAPDH was included as a loading control. c, d The mRNA expression levels of the SW1990 and PANC-1 cells determined by qRT-PCR showed the same trend as the western blot results. (* indicates a significant difference by one-way ANOVA, *P < 0.05, **P < 0.001)