Fig. 1

Endothelial cell monolayer permeability assays using FITC-dextran. a) Relative permeability 4 h after irradiation, compared to non-irradiated controls (0 Gy). b) Relative permeability of cell monolayers measured 24 h after irradiation with 4 Gy, after treatment with VEGF-A (100 ng/ml) or TNFα (100 ng/ml) for 24 h, and after exposure to APMA (10 ng/ml) for 2 h, compared to vehicle (DMSO, 0.1%) only-treated controls. c) Effects of ADAM inhibitors GI254023X (10 μM; specific for ADAM10 only) and GW280264X (10 μM; inhibits both ADAM10 and ADAM17). Inhibitor or vehicle were added to the monolayers 24 h before measurement. d) ADAM inhibitors counteract the irradiation-induced increase in permeability. Measurements were performed 24 h after addition of inhibitors and 4 h (left) or 24 h (right) after irradiation, respectively. Data shown are means (n ≥ 3) and standard deviations. Statistics: t-test, **p < 0.01, ***p < 0.001