Fig. 4

Dnmt3a R878H and Nras G12D-induced leukemia is transplantable with advantage of myeloid proliferation. a The percentage of CD45.2⁺ cells in PB of each group after BMT. (Left panel) Fold line diagram of the change of CD45.2⁺ cells. (Right panel) Representative flow cytometric results. b Count of WBCs in PB of each group 24 weeks after BMT. c Morphological analysis of BM cells with Wright’s staining of each group. d Macroscopic appearance of spleen of each group at the same time point. e Flow cytometric analysis of CD45.2⁺ cells and hematopoietic cell populations in BM of each group. CD45.2⁺ cells were gated for analysis of Gr-1⁺, B220⁺, CD3⁺ and c-Kit⁺ cells. n = 5 for each group. Horizontal bars correspond to Mean ± SEM values. * P < 0.05, ** P < 0.01, ***P < 0.001, significance was determined by two-tailed Student’s t test