Fig. 5
From: Overexpression of iASPP is required for autophagy in response to oxidative stress in choriocarcinoma
Autophagy inhibition sensitized choriocarcinoma cells in response to hydrogen peroxide. a Chloroquine inhibition on autophagy per se had no effect on the viability of choriocarcinoma cells as measured by MTT assay in the first 24 h. b Addition of chloroquine (20 μM) enhanced the toxic effect of hydrogen peroxide at low dosage after 24 h (*P < 0.001; #P = 0.02). c Choriocarcinoma cells with iASPP knockdown (siiaspp#1/2) were more sensitive to hydrogen peroxide than scramble control (si). d Change of LC3 expression after addition of hydrogen peroxide (H2, 0.3 mM) with or without chloroquine (C, 20 μM). (** P < 0.05)