Fig. 3
From: Overexpression of iASPP is required for autophagy in response to oxidative stress in choriocarcinoma
Knockdown of iASPP induced senescence in choriocarcinoma cells. a The presence of senescent cells was detected by SA-β-Galactosidase staining (blue color, indicated with arrows) in both JEG-3 and JAR cells with iASPP knockdown (200X magnification). b The percentage of SA-β-Galactosidase positive cells was measured and compared. Statistically significant increase in senescence was found in JEG-3, si vs siiaspp#1 (P = 0.04), and JAR, si vs siiaspp#2 (P = 0.005), respectively. c Increase in p21WAF1/Cip1 mRNA levels in JEG-3 and JAR cells with iASPP knockdown as measured by qRT-PCR. d At protein level, knockdown of iASPP also induced the expression of p21WAF1/Cip1 in choriocarcinoma cells