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Table 1 Transfection efficiency (TE), mean fluorescence intensity (MFI), apoptosis rate (AR) and necrosis rates (NR) of HepG2 cells analyzed by flowcytometry in vitro: pDNA (A),U-nanoplex (B), Ca-nanoplex (C), Pll--nanoplex (D), L-nanoplex (E). All the data were calculated with one-way analysis of variance and Fisher-LSD multiple comparison tests

From: Synergism of wt-p53 and synthetic material in local nano-TAE gene therapy of hepatoma: comparison of four systems and the possible mechanism

 

Group A

Group B

Group C

Group D

Group E

TE (%)

12hs

0

0.1 ± 0.05

0.1 ± 0.10

0.1 ± 0.08

0.7 ± 0.10a

36hs

0

0.1 ± 0.06

0.3 ± 0.08

2.1 ± 0.26a,b,c

20.1 ± 1.53a,b,c,d

72hs

0

0.2 ± 0.02

0.4 ± 0.14

6.3 ± 0.33a,b,c

16.8 ± 1.48a,b,c,d

MFI

12hs

86.4 ± 7.22

88.0 ± 5.61

89.5 ± 2.70

96.5 ± 16.00

93.8 ± 3.56

36hs

90.3 ± 2.80

86.3 ± 5.59

93.3 ± 3.77

106.7 ± 10.49a,b,c

189.9 ± 10.03a,b,c,d

72hs

85.4 ± 2.68

97.2 ± 4.62

95.3 ± 3.53

135.4 ± 17.10a,b,c

143.2 ± 17.66a,b,c,d

AR (%)

36hs

0.2 ± 0.08

5.0 ± 1.47a

0.4 ± 0.06b

6.5 ± 0.71a,b,c

2.0 ± 0.57a,b,c,d

72hs

1.7 ± 0.58

2.5 ± 0.75

1.85 ± 0.28

36.0 ± 1.70a,b,c

24.6 ± 1.93a,b,c,d

NR (%)

36hs

0.8 ± 0.17

1.7 ± 0.48

1.0 ± 0.06

6.8 ± 0.64a,b,c

9.8 ± 3.38a,b,c,d

72hs

2.1 ± 0.41

3.2 ± 0.89

2.6 ± 0.41

15.3 ± 4.08a,b,c

18.0 ± 10.92a,b,c,d

  1. a,b,c,drepresent significant difference from group A, B, C, D respectively