Fig. 3

BMSCs suppress miR-101-3p which directly targets BIRC5. a MM.1S cells were cultured for 24 h in the following settings: cultured alone with or without 5 nM BTZ, co-cultured with HS-5 cells with or without 5 nM BTZ, co-cultured with HS-5 cells pre-treated with BFA or separated with a TW insert. Cells from all conditions were harvested, cDNAs isolated and used in real time PCR for analysis of BIRC5 mRNA expression. Analyzed data are from two separate experiments, *p < 0.05, **p < 0.01, ***p < 0.001. b MM cells were transduced with viral particles of miR-101-3p or negative control, harvested after puromycin selection of eGFP⁺ population to be examined in real time PCR for confirmation of transduction. c MM cells with overexpressed miR-101-3p were harvested and the cDNAs were applied to real time PCR for measuring BIRC5 mRNA level. d MM cells with overexpressed miR-101-3p were harvested and lysates were used in WB to detect survivin protein. e The HEK-293 T cells were transiently co-transfected with the mutant or wild type BIRC5-UTR luciferase reporter vectors together with miR-101 expressing or control vectors. Relative luciferase activities were analyzed 48 h after transfection