Fig. 2

CD171-specific CAR-T cells recognize and kill retinoblastoma cell lines in vitro. a Scheme of CD171-specific 2nd generation CAR variants displaying color-coding of the different constructs used. b IFNG and IL2 release of CAR-T cells following a co-culture at a 2:1 effector:target (E:T) ratio with retinoblastoma cell lines (mean ± SD, n = 3). Cell surface expression of activation markers CD137 and CD25 c and inhibitory receptors LAG3, PD1 and TIM3 (d) on CAR-T cells following a 24-h tumor co-culture at a 2:1 E:T ratio (mean ± SD, n = 3). Color-coding is the same as used in B. e Cytotoxicity of CD171-specific CAR-T cells is determined by luciferase-based killing assay following a tumor co-culture for 24, 48 and 72 h at a 2:1 E:T ratio (mean ± SD, n = 3). Color-coding is the same as used in B. * p ≤ 0.5; **, p ≤ 0.01