Fig. 3

Spontaneous/idiopathic mitotic catastrophe (MC) in primary glioblastoma cultures. Polynucleated cell following MC in GB6 in early monolayer culture conditions (a) as well as abnormal mitoses in early passages of NSC-like conditions in GB9 are visible; (b-c) Multipolar spindles in GFAP positive cell abnormal mitosis (b) as well as polynuclear cells with asymmetric distribution of phosphorylated histone 3 may be easily detected in early passages of GB 7 in NSC-like conditions (c); The percentage of cells with the features of mitotic catastrophe (abnormal metaphases with spindle apparatus/chromosomal misalignments, mono- and multipolar spindles, bi- and multinuclear cells formed following MC) differs depending on case (GB6 and GB9) in the same conditions, here NSC-like ones; (d) Cells with the features of MC may be simultaneously SA-β-Gal positive or pass through mitotic cell death, as polynucleated senescent cell in GB9 in passage 4 of NSC-like conditions (e-f). The average percentage was obtained by analyzing of at least 200 cells per case (GB6 and GB9). Error bars indicate SEM