Fig. 7

The lentivirus-mediated CDglyTK gene by intra-artery perfusion causes no discernable systemic toxic effects in vivo. a Reverse transcriptase polymerase chain reaction analysis of CD/TK fusion gene expression in the underlying flap bed and major internal organs. Analysis of serum b ALT and c AST over a 42-day post-operative period. d Assessment of the inflammatory response in major internal organs in the experimental group by hematoxylin and eosin (H&E) staining. Scale bar: 100 μm. Data was shown as the mean ± SD of six independent samples. LV-CDglyTK, flaps transfected with lentivirus-mediated CDglyTK gene; LV-GFP, flaps transfected with empty lentivirus; Control, non-transfected flaps