Fig. 6

The apoptosis assay in vivo tumor tissues caused by active drug converted from prodrug using TUNEL assay kit. a Cell apoptosis assay of the transplanted tumors by TUNEL assay. Red arrowhead indicated the positive apoptosis cells in the tumor tissue. b The percentage of apoptotic cells in tumor tissue was analyzed by Image-pro plus 6.0. Data was shown as the mean ± SD of six independent samples. Scale bar: 50 μm. *P < 0.05; **P < 0.01. LV-CD/TK, flaps transfected with lentivirus-mediated CDglyTK gene; LV-GFP, flaps transfected with empty lentivirus; Control, non-transfected flaps