Fig. 3

Effects of the double suicide CD/TK on the selective killing efficacy on SHZ-88 cells. a The cytotoxic effects of ganciclovir (GCV) and 5-flucytosine (5-FC) to transgenic cells. b The cytotoxic effects of different prodrugs to transgenic cells. c Cell apoptosis assay of 5-FC + GCV on transgenic cells. d Cell cycle analysis of 5-FC + GCV on transgenic cells. e Bystander effect analysis of the CD/TK fusion gene and prodrug system on SHZ-88 cells. Data are expressed as the mean ± SD, n = 3. & P < 0.001 compared to the LV-GFP group; #P < 0.001 compared to the control group; **P < 0.01; ***P < 0.001; LV-CD/TK, CDglyTK fusion gene transfected group; LV-GFP, empty lentivirus transfected group; Control, the untransfected group. Three replicates were done in each experiment