Fig. 4

Peptide binding affinities of putative H2^b-restricted NY-BR-1-specific CTL epitopes. Peptide binding affinity of putative CTL epitopes to H2 K^b and –D^b molecules was tested on RMA-S cells using a H2-D^b-specific monoclonal antibody B22.249 or b H2-K^b-specific monoclonal antibody E3–25. Surface expression of MHC I molecules stabilized by external addition of synthetic candidate epitopes was measured by flow cytometry to provide an estimate of peptide binding affinity. The H2-D^b-restricted E7-specific epitope E7_49–57 and the H2-K^b-restricted OVA-specific epitope OVA_257–264 served as positive controls