Fig. 4
From: Response of neuroblastoma cells to RF currents as a function of the signal frequency
Immunofluorescence. a Immunofluorescence for p53, Bax and caspase-3 at 12 h or 24 h of sham- or CRET-treatment: representative micrographs. Alexa Red for p53, Alexa Green for Bax and caspase-3, and Hoechst 33258 for DNA. Bottom line displays merged images of caspase 3+ (green) and Hoechst (blue). C = Control, T = Treated with 448 kHz at 50 μA/mm2. Bar = 100 μm. b Quantification of p53, Bax and caspase-3 positive cells. Values are means ± SEM of at least 3 experimental replicates, normalized over sham-exposed controls. *: 0.05 > p ≥ 0.01; **: 0.01 > p ≥ 0.001; ***: p < 0.001; Student’s t-test