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Fig. 1 | BMC Cancer

Fig. 1

From: Inhibitory short peptides targeting EPS8/ABI1/SOS1 tri-complex suppress invasion and metastasis of ovarian cancer cells

Fig. 1

The formation of endogenous SOS1/EPS8/ABI1 tri-complex in ovarian cancer cell invasion. ABI1 served as a scaffold protein in SOS1/EPS8/ABI1 tri-complex. a. The metastatic ovarian cancer cell line SK-OV3 was treated with LPA for 5 min to induce cell invasion. Co-IP assay was employed to investigate the formation of endogenous SOS1/EPS8/ABI1 tri-complex after LPA stimulation. b. The Myc-tagged EPS8, HA-tagged ABI1 and Flag-tagged SOS1 recombinant plasmids were constructed. c. The OVCAR3 cells, which has no ABI1 expression, were infected by every two of the three expression plasmids (Myc-EPS8, HA-ABI1 and Flag-SOS1). Cell lysates were collected after LPA stimulation. The Co-IP assay was used to detect the interactions of ABI1-SOS1, ABI1-EPS8, and SOS1-EPS8

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BMC Cancer

ISSN: 1471-2407

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