Skip to main content

Advertisement

Table 2 Statistical characteristics for the study genes and selected antibodies and corresponding optimized antibody dilution factors for IHC experiment. Twenty-seven genes are listed in view of their respective HR, 95% CI, P value, C-index and log2 ratio. MTUS1 and COL3A1 were selected in view of the findings in our previous work. An optimized dilution factor for IHC analysis could be determined for 12 of the 29 proteins. All antibodies with determined optimized dilution were polyclonal antibodies except the antibody for PTEN which was monoclonal

From: Immunohistochemical validation of COL3A1, GPR158 and PITHD1 as prognostic biomarkers in early-stage ovarian carcinomas

Gene symbol Histotype Survival HR 95% CI P Value C-index Log2 ratio Antibody Company Optimized dilution
ARHGAP21 EC OS 1.00 1.00–1.003 0.0011 0.80 1.53 22,183–1-AP Nordic Biosite 1:50
ARMC3 HGSC OS 0.80 0.70–0.92 0.0013 0.64 −3.83 HPA037823 Sigma-Aldrich -
C7 EC OS 1.32 1.03–1.69 0.0310 0.72 5.55 HPA001465 Sigma-Aldrich -
CDH18 HGSC OS 1.18 1.06–1.32 0.0020 0.64 5.67 HPA014416 Sigma-Aldrich -
CES3 EC OS 0.66 0.49–0.90 0.0088 0.74 −4.17 HPA041008 Sigma-Aldrich 1:500
COL11A1 CCC OS 1.49 1.13–1.96 0.0042 0.77 4.32 ab64883 Abcam -
EHD3 CCC DSS 11.67 2.23–59.35 0.0031 0.95 2.50 HPA049890 Sigma-Aldrich -
FRMPD2 EC OS 0.76 0.60–0.96 0.0228 0.71 −5.34 HPA045059 Sigma-Aldrich -
GABRP CCC OS 1.34 1.03–1.73 0.0275 0.71 5.05 PA5–46830 Thermo Fisher -
GID4 EC OS 1.04 1.02–1.07 0.0013 0.79 0.75 HPA044348 Sigma-Aldrich 1:150
GPR158 MC OS 1.44 1.02–2.04 0.0380 0.77 4.93 HPA013185 Sigma-Aldrich 1:25
GRM5 EC OS 0.56 0.35–0.91 0.0179 0.73 −4.17 ab76316 Abcam -
IGHG1 CCC DSS 1.20 1.04–1.39 0.0128 0.72 7.40 SAB1401207 Sigma-Aldrich 1:25
JCHAIN CCC OS 1.23 1.04–1.46 0.0133 0.73 6.34 HPA044132 Sigma-Aldrich -
KIF26B EC DSS 0.46 0.23–0.91 0.0248 0.91 −3.68 HPA027709 Sigma-Aldrich -
MAP7D2 MC OS 1.86 1.09–3.16 0.0218 0.83 4.06 HPA051508 Sigma-Aldrich -
MTRNR2L1 HGSC OS 1.23 1.08–1.40 0.0017 0.64 5.68 HPA059729 Sigma-Aldrich -
MUC15 EC OS 0.66 0.50–0.87 0.0032 0.79 −5.33 HPA026110 Sigma-Aldrich -
PITHD1 CCC DSS 146.53 6.19–3470 0.0020 0.89 0.57 PAB20914 Abnova 1:50
PTEN EC DSS 404.90 1.23–133,809 0.0425 0.96 0.99 ab109454 Abcam 1:100
RTKN2 MC OS 7.87 1.48–41.96 0.0157 0.89 1.66 17,458–1-AP Nordic Biosite 1:25
SLC9A4 MC OS 0.65 0.44–0.96 0.0292 0.76 −4.65 HPA036096 Sigma-Aldrich -
SMYD2 CCC DSS 56.79 3.92–822.37 0.0031 0.89 0.91 PA5–51339 Thermo Fisher -
TRIM71 HGSC DSS 0.78 0.66–0.92 0.0036 0.67 −4.30 HPA038141 Sigma-Aldrich -
TRIO CCC DSS 19.72 2.47–157.47 0.0049 0.93 1.27 HPA008157 Sigma-Aldrich 1:25
TTK MC OS 5.76 1.52–21.83 0.0100 0.88 1.98 PAB3320 Abnova 1:25
VNN1 CCC OS 1.36 1.05–1.75 0.0184 0.71 4.48 HPA064145 Sigma-Aldrich -
COL3A1 95 RNA-seq samples OS - - - - - HPA007583 Sigma-Aldrich 1:50
MTUS1 95 RNA-seq samples OS - - - - - ab198176 Abcam 1:40