Skip to main content

Table 2 Statistical characteristics for the study genes and selected antibodies and corresponding optimized antibody dilution factors for IHC experiment. Twenty-seven genes are listed in view of their respective HR, 95% CI, P value, C-index and log2 ratio. MTUS1 and COL3A1 were selected in view of the findings in our previous work. An optimized dilution factor for IHC analysis could be determined for 12 of the 29 proteins. All antibodies with determined optimized dilution were polyclonal antibodies except the antibody for PTEN which was monoclonal

From: Immunohistochemical validation of COL3A1, GPR158 and PITHD1 as prognostic biomarkers in early-stage ovarian carcinomas

Gene symbol

Histotype

Survival

HR

95% CI

P Value

C-index

Log2 ratio

Antibody

Company

Optimized dilution

ARHGAP21

EC

OS

1.00

1.00–1.003

0.0011

0.80

1.53

22,183–1-AP

Nordic Biosite

1:50

ARMC3

HGSC

OS

0.80

0.70–0.92

0.0013

0.64

−3.83

HPA037823

Sigma-Aldrich

-

C7

EC

OS

1.32

1.03–1.69

0.0310

0.72

5.55

HPA001465

Sigma-Aldrich

-

CDH18

HGSC

OS

1.18

1.06–1.32

0.0020

0.64

5.67

HPA014416

Sigma-Aldrich

-

CES3

EC

OS

0.66

0.49–0.90

0.0088

0.74

−4.17

HPA041008

Sigma-Aldrich

1:500

COL11A1

CCC

OS

1.49

1.13–1.96

0.0042

0.77

4.32

ab64883

Abcam

-

EHD3

CCC

DSS

11.67

2.23–59.35

0.0031

0.95

2.50

HPA049890

Sigma-Aldrich

-

FRMPD2

EC

OS

0.76

0.60–0.96

0.0228

0.71

−5.34

HPA045059

Sigma-Aldrich

-

GABRP

CCC

OS

1.34

1.03–1.73

0.0275

0.71

5.05

PA5–46830

Thermo Fisher

-

GID4

EC

OS

1.04

1.02–1.07

0.0013

0.79

0.75

HPA044348

Sigma-Aldrich

1:150

GPR158

MC

OS

1.44

1.02–2.04

0.0380

0.77

4.93

HPA013185

Sigma-Aldrich

1:25

GRM5

EC

OS

0.56

0.35–0.91

0.0179

0.73

−4.17

ab76316

Abcam

-

IGHG1

CCC

DSS

1.20

1.04–1.39

0.0128

0.72

7.40

SAB1401207

Sigma-Aldrich

1:25

JCHAIN

CCC

OS

1.23

1.04–1.46

0.0133

0.73

6.34

HPA044132

Sigma-Aldrich

-

KIF26B

EC

DSS

0.46

0.23–0.91

0.0248

0.91

−3.68

HPA027709

Sigma-Aldrich

-

MAP7D2

MC

OS

1.86

1.09–3.16

0.0218

0.83

4.06

HPA051508

Sigma-Aldrich

-

MTRNR2L1

HGSC

OS

1.23

1.08–1.40

0.0017

0.64

5.68

HPA059729

Sigma-Aldrich

-

MUC15

EC

OS

0.66

0.50–0.87

0.0032

0.79

−5.33

HPA026110

Sigma-Aldrich

-

PITHD1

CCC

DSS

146.53

6.19–3470

0.0020

0.89

0.57

PAB20914

Abnova

1:50

PTEN

EC

DSS

404.90

1.23–133,809

0.0425

0.96

0.99

ab109454

Abcam

1:100

RTKN2

MC

OS

7.87

1.48–41.96

0.0157

0.89

1.66

17,458–1-AP

Nordic Biosite

1:25

SLC9A4

MC

OS

0.65

0.44–0.96

0.0292

0.76

−4.65

HPA036096

Sigma-Aldrich

-

SMYD2

CCC

DSS

56.79

3.92–822.37

0.0031

0.89

0.91

PA5–51339

Thermo Fisher

-

TRIM71

HGSC

DSS

0.78

0.66–0.92

0.0036

0.67

−4.30

HPA038141

Sigma-Aldrich

-

TRIO

CCC

DSS

19.72

2.47–157.47

0.0049

0.93

1.27

HPA008157

Sigma-Aldrich

1:25

TTK

MC

OS

5.76

1.52–21.83

0.0100

0.88

1.98

PAB3320

Abnova

1:25

VNN1

CCC

OS

1.36

1.05–1.75

0.0184

0.71

4.48

HPA064145

Sigma-Aldrich

-

COL3A1

95 RNA-seq samples

OS

-

-

-

-

-

HPA007583

Sigma-Aldrich

1:50

MTUS1

95 RNA-seq samples

OS

-

-

-

-

-

ab198176

Abcam

1:40

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us