Fig. 7

UXT regulates human renal cancer cell proliferation, migration and invasion via EZH2. a 786-O cells were infected with different combinations of lentivirus as indicated. 72 h after infection, CCK8 assay was utilized to quantify cell viability at each time point. Data are plotted as the mean ± SD of 3 independent experiments. Western blot analysis of the cells was performed to evaluate the expression of EZH2. GAPDH is used as an internal control. b After infection as (a), after infection, colony formation assay was performed to estimate the anchorage-independent growth ability of 786-O cells. Images were taken at 2 weeks after 2 weeks (1). The number of colonies was quantified (2). c After infection as (a), cells were used for Transwell assays. Representative photographs were taken at × 200 magnification (1). Number of migrated and invaded cells was quantified in 4 random images from each treatment group. Results are the mean ± SD from 3 independent experiments plotted as percent (%) migrating and invading cells relative to sh-LacZ treatment