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Fig. 5 | BMC Cancer

Fig. 5

From: Ubiquitous expressed transcript promotes tumorigenesis by acting as a positive modulator of the polycomb repressive complex 2 in clear cell renal cell carcinoma

Fig. 5

UXT promotes EZH2 HMTase activity. a Real-time PCR analysis of UXT mRNA showed efficient UXT knockdown by shRNA expression in 786-O and EZH2-knockout 786-O cells. b, c Real-time quantitative PCR shows that UXT knockdown promotes the expression of endogenous EZH2 target genes (HOXA9 and DAB2IP) in EZH2 WT cells, but not in EZH2-knockout cells. Data are plotted as the mean ± SD of three independent experiments. d, e Western blot analysis shows upregulation of DAB2IP and HOXA9 and downregulation of histone H3K27me3 in EZH2 WT 786-O cell lines (D), but not in EZH2-knockout cells(E). Total histone H3 and GAPDH were shown as control. f ChIP–qPCR analysis of H3K27me3 enrichment on HOXA9 gene promoter in UXT knockdown cells. UXT positive regulates H3K27me3 levels at PRC2 target loci in 786-O cells

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BMC Cancer

ISSN: 1471-2407

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