Fig. 4

MiR-6778-5p participates in the regulation of cell proliferation by targeting YWHAE. a Data mining for YWHAE transcription using the datasets from the Oncomine database. The datasets showed a decreased level of YWHAE mRNA in CRC tissues compared with control tissues. The Y value represents log2 median-centered ratio (normalized expression). b-c The expression of YWHAE in colon epithelial cells NCM460 and colon cancer cells HCT116, HT29 and SW620 measured by real-time PCR and western blot. d The expression of YWHAE in HCT116 cells was measured by western blot after siRNA or CRISPR activation plasmid transfection. GAPDH was used as a control. e Viability of HCT116 cells was measured using CCK-8 assay at 24 h after transfection. f GSEA enrichment plots indicated that BIOCARTA_P53_PATHWAY and KEGG_P53_PATHWAY gene sets preferentially enriched in YWHAE^high samples. NES, normalized enrichment score; FDR, false discovery rate; P-Val, nominal P value. g The expression correlation between YWHAE and p53 in CRC was analyzed by the cBioPortal database. h Functional protein association networks of the p53 pathway was analyzed by STRING10.5. i The expression of p53, Bax, Bcl-2 in HTC116 cells was measured by western blot under the corresponding treatments. GAPDH was used as a control. Data are represented as mean ± SD. *P < 0.05, **P < 0.01 versus the control group